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Construction and physiological studies of hydrogenase depleted mutants of Desulfovibrio fructosovorans

Authors :
Casalot, Laurence
Valette, Odile
De Luca, Gilles
Dermoun, Zorah
Rousset, Marc
de Philip, Pascale
Source :
FEMS Microbiology Letters. Aug2002, Vol. 214 Issue 1, p107. 6p.
Publication Year :
2002

Abstract

Desulfovibrio fructosovorans possesses two periplasmic hydrogenases (a nickel-iron and an iron hydrogenase) and a cytoplasmic NADP-dependent hydrogenase. The hydAB genes encoding the periplasmic iron hydrogenase were replaced, in the wild-type strain as well as in single mutants depleted of one of the other two hydrogenases, by the acc1 gene encoding resistance to gentamycin. Molecular characterization and remaining activity measurements of the resulting single and double mutants were performed. All mutated strains exhibited similar growth when H2 was the electron donor but they grew differently on fructose, lactate or pyruvate as electron donors. Our results indicate that the loss of one enzyme might be compensated by another even though hydrogenases have different localization in the cells. [Copyright &y& Elsevier]

Details

Language :
English
ISSN :
03781097
Volume :
214
Issue :
1
Database :
Academic Search Index
Journal :
FEMS Microbiology Letters
Publication Type :
Academic Journal
Accession number :
7865529
Full Text :
https://doi.org/10.1111/j.1574-6968.2002.tb11332.x