High-solids loading enzymatic hydrolysis of waste papers for biofuel production

Abstract: Waste papers (newspaper, office paper, magazines and cardboard in this study) with 50–73% (w/w oven dry weight) carbohydrate contents have considerable potential as raw materials for bioethanol production. A particle size reduction step of wet blending prior to enzymatic hydrolysis of newspaper was found to increase the glucan conversion efficiency by up to 10%. High-solids loading hydrolysis at 15% (w/w) of four types of paper using two enzyme alternatives, Celluclast 1.5L supplemented with Novozyme 188 and Cellic Ctec 1 (Novozymes A/S, Demark), at various enzyme concentrations were successfully performed in a lab-scale overhead-stirred reactor. This work has identified the relative saccharification performance for the four types of paper and shows office paper and cardboard to be more suitable for producing bioethanol than newspaper or magazine paper. The experimental data were also very well described by a modified, simple three parameter glucan and xylan hydrolysis model. These findings provide the possibility for incorporating this validated kinetic model into process designs required for commercial scale bioethanol production from waste paper resources. [Copyright &y& Elsevier]