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Comparison of Anti-Hyperglycemic Activities Between Low- and High-Degree of Polymerization Procyanidin Fractions from Cacao Liquor Extract.
- Source :
-
Journal of Food & Drug Analysis . Apr2012 Supplement, Vol. 20, p283-287. 5p. 1 Chart, 2 Graphs. - Publication Year :
- 2012
-
Abstract
- Procyanidins are thought to be effective for prevention of hyperglycemia. To clarify the degree of polymerization (DP) of procyanidins on anti-hyperglycemic effects, we prepared low-DP (DP S 3) and high-DP (DP > 4) procyanidin fractions from cacao liquor, and examined whether these procyanidin fractions prevent hyperglycemia in vitro. We found that both fractions promoted glucose uptake in L6 myotubes in a dose-dependent manner accompanied by translocation of GLUT4 to the plasma membrane. Moreover, both fractions stimulate phosphorylation of AMP-activated protein kinase (AMPK). In these beneficial effects, low-DP fraction was more effective than high-DP one. On the other hand, high-DP fraction showed greater inhibitory effect of intestinal a-glucosidase activity than low-DP fraction in a cell free-incubation system. In conclusion, cacao liquor procyanidins have anti-hyperglycemic activities and low-DP procyanidins mainly contribute to stimulate glucose uptake by glucose transporter 4 translocation through AMPK-dependent pathway in skeletal muscle while high-DP procyanidins mainly contributes to inhibit the a-glucosidase activity in small intestine. [ABSTRACT FROM AUTHOR]
- Subjects :
- *FLAVONOIDS
*HYPERGLYCEMIA prevention
*TANNINS
*MASS spectrometry methodology
*LIQUID chromatography
*ANIMAL experimentation
*BIOCHEMISTRY
*BIOPHYSICS
*CACAO
*CARRIER proteins
*CELL culture
*DOSE-effect relationship in pharmacology
*GLUCOSE
*GLYCOSIDASES
*HIGH performance liquid chromatography
*HYPERGLYCEMIA
*PHENOMENOLOGY
*RESEARCH methodology
*PROTEIN kinases
*RATS
*STATISTICS
*WESTERN immunoblotting
*PLANT extracts
*DATA analysis
*DESCRIPTIVE statistics
*CHEMICAL inhibitors
*THERAPEUTICS
Subjects
Details
- Language :
- English
- ISSN :
- 10219498
- Volume :
- 20
- Database :
- Academic Search Index
- Journal :
- Journal of Food & Drug Analysis
- Publication Type :
- Academic Journal
- Accession number :
- 78368556