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HPLC measurement of harderoporphyrin in the harderian glands of rodents as a biomarker for sub-lethal or chronic arsenic exposure
- Source :
-
Toxicology Letters . Jul2002, Vol. 133 Issue 1, p93. 9p. - Publication Year :
- 2002
-
Abstract
- An improved HPLC method has been established for the measurement of harderoporphyrin (HP) in the harderian gland of rats and mice. Groups of female Wistar rats were given a single oral dose of sodium arsenite at 0, 0.5 or 5.0 mg As(III)/kg body weight, or a slurry of arsenic-contaminated soil at equivalent dose rates and the animals were sacrificed 96 h after dosing. A group of C57BL/6J female mice were chronically exposed to drinking water containing 500 μg As(V)/l of sodium arsenate ad libitum for over 2 years. Porphyrins were measured in the harderian glands of rats and mice. Our results suggest that HP and the alteration of the porphyrin profile in the harderian glands of rodents is a highly sensitive biomarker for both single sub-lethal and chronic arsenic exposure. [Copyright &y& Elsevier]
- Subjects :
- *PORPHYRINS
*HIGH performance liquid chromatography
*BIOMARKERS
*LABORATORY rodents
Subjects
Details
- Language :
- English
- ISSN :
- 03784274
- Volume :
- 133
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Toxicology Letters
- Publication Type :
- Academic Journal
- Accession number :
- 7826376
- Full Text :
- https://doi.org/10.1016/S0378-4274(02)00089-9