Immunity 12 years after alemtuzumab in RA: CD5+ B-cell depletion, thymus-dependent T-cell reconstitution and normal vaccine responses.

Objectives. Lymphocyte depleting therapies have been used to treat refractory autoimmune disease, including RA, but treatment may be associated with long-term lymphopenia. It is unclear whether delayed reconstitution preferentially affects lymphocyte subsets, how this modulates immune challenges and whether thymic function influences the outcome. These questions are now addressed in a detailed analysis of RA patients 12 years after alemtuzumab (anti-CD52) treatment.Methods. Blood was obtained from 20 RA patients 12 years after alemtuzumab treatment. Lymphocyte subsets were enumerated by flow cytometry. T-cell receptor excision circles (TRECs)/ml were determined to quantify thymic function, and serological responses to neoantigens and recall antigens were assessed.Results. RA patients remained lymphopenic 12 years after their first dose of alemtuzumab. CD5+ B cells, which may be associated with autoantibody production, were significantly reduced in alemtuzumab-treated patients compared with age-matched disease controls. In addition, naïve and memory CD4+ T-cell subsets were present in altered proportions in patients who had received alemtuzumab, with increased effector memory CD4+ T cells, and decreased naïve and central memory CD4+ T cells. TRECs were detectable in alemtuzumab-treated patients and correlated with CD4+ lymphocyte counts. Vaccine responses to neoantigens and recall antigens fell within the normal range for an ageing population.Conclusions. Alemtuzumab therapy resulted in long-term alterations in lymphocyte subsets. The significance of these changes remains uncertain but patients respond normally to antigenic challenges. Thymic function remains an important determinant of T-cell reconstitution even several years after lymphocytotoxic therapy. [ABSTRACT FROM AUTHOR]