LC DETERMINATION OF NATAMYCIN IN DOOGH WITH UV DETECTION.

A rapid and simple High-Performance Liquid Chromatographic method with UV detection (HPLC-UV) has been developed for the first time to determination of natamycin in Doogh. Doogh is a refreshing drink that is made with yogurt, water, and mint. The method is based on extraction of 10 g of Doogh with 75 ml. of methanol and 25 ml. of distilled water. An aliquot of 20 p1 of Extract after filtration injected to HPLC. This compound was detected using a Grace Smart PP C18 column (100 × 4.6 mm, 3 μm I.D.) and the mobile phase used, was acetonitril: 30mM perchloric acid (35:65) in 5 mm, The flow rate was maintained at 0.5 rnL/min and the analytical signal was measured at 304 nm. [ABSTRACT FROM AUTHOR]