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<atl>Simultaneous determination of ammonia, dimethylamine, trimethylamine and trimethylamine-n-oxide in fish extracts by capillary electrophoresis with indirect UV-detection
- Source :
-
Food Chemistry . Apr2002, Vol. 76 Issue 4, p509. 10p. - Publication Year :
- 2002
-
Abstract
- A capillary electrophoretic method with indirect UV detection is described for simultaneous determination of ammonia, dimethylamine (DMA), trimethylamine (TMA) and trimethylamine-n-oxide (TMAO) in aqueous extracts of fish. A buffer consisting of 4 mM formic acid, 5 mM copper(II)sulfate and 3 mM crown ether 18-crown-6 enabled separation of the analytes in 5–10 min. The use of an extended light path capillary technique resulted in a good sensitivity and repeatability. The linear dynamic range, based on a hydrostatic injection at 50 mbar for 2 s, was from the detection limit to at least 2.5 mM. The detection limit for ammonia, DMA, TMA, and TMAO was less than 0.04 mM, corresponding to 2 mg nitrogen per 100 g fish. As an extra benefit, the method also provided a quantitative determination of potassium, sodium, calcium and magnesium ions. [Copyright &y& Elsevier]
- Subjects :
- *FISHES
*CAPILLARY electrophoresis
*AMINES
*DIMETHYLAMINE
Subjects
Details
- Language :
- English
- ISSN :
- 03088146
- Volume :
- 76
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Food Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 7756190
- Full Text :
- https://doi.org/10.1016/S0308-8146(01)00289-8