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Amperometric Immunosensor Based on L-Cysteine/Gold Colloidal Nanoparticles for Carbofuran Detection.

Authors :
Du, Shuyuan
Wang, Xiangyou
Sun, Xia
Li, Qingqing
Source :
Analytical Letters. Jul2012, Vol. 45 Issue 10, p1230-1241. 12p.
Publication Year :
2012

Abstract

In this study, anti-carbofuran monoclonal antibodies (Ab) were immobilized onto a gold electrode surface modified with multilayers of L-cysteine and gold colloidal nanoparticles (GNPs). Furthermore, horseradish peroxidase (HRP) as enzyme membrane was used for blocking unspecific sites and amplifying signal. The conformational properties of the immunosensor were characterized using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The concentration of antibody solution, pH of working buffer and incubation time were studied in detail for optimization of analytical performance. Under optimal conditions, the variation of current response was proportional to the concentration of carbofuran which ranged from 0.01 ng/mL to 50 ng/mL with a correlation coefficient of 0.9912. The detection limit was 0.01 ng/mL (S/N = 3). The proposed immunosensor exhibited good reproducibility and stability and it can be used for the rapid detection of carbofuran pesticide. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00032719
Volume :
45
Issue :
10
Database :
Academic Search Index
Journal :
Analytical Letters
Publication Type :
Academic Journal
Accession number :
77492622
Full Text :
https://doi.org/10.1080/00032719.2012.673105