Back to Search
Start Over
Response of in vivo protein synthesis in T lymphocytes and leucocytes to an endotoxin challenge in healthy volunteers.
- Source :
-
Clinical & Experimental Immunology . Nov2002, Vol. 130 Issue 2, p263-270. 8p. - Publication Year :
- 2002
-
Abstract
- Summary In vivo determination of protein synthesis in immune cells reflects metabolic activity and immunological activation. An intravenous injection of endotoxin to healthy volunteers was used as a human sepsis model, and in vivo protein synthesis of T lymphocytes and leucocytes was measured. The results were related to plasma concentrations of selected cytokines, peripheral cell counts and subpopulations of immune cells. The subjects (n = 8 + 8) were randomized to an endotoxin (4 ng/kg) or a saline group. In vivo protein synthesis was determined twice: before and 1–2·5 h after the endotoxin/saline injection. Protein synthesis decreased in isolated T lymphocytes, but increased in leucocytes. Plasma levels of TNF-α , IL-8, IL-6, IL-1 ra and IL-10 were elevated, whereas IL-2 and IFN-γ , produced predominantly by T lymphocytes, did not change in response to endotoxin. Neutrophils increased, whereas lymphocytes and monocytes decreased 2·5 h after the endotoxin injection. Flow cytometry revealed a drop in total CD3+ T lymphocytes and CD56+ natural killer cells, accompanied by an increase in CD15+ granulocytes. In summary, in vivo protein synthesis decreased in T lymphocytes, while the total leucocyte population showed a concomitant increase immediately after the endotoxin challenge. The changes in protein synthesis were accompanied by alterations in immune cell subpopulations and in plasma cytokine levels. [ABSTRACT FROM AUTHOR]
- Subjects :
- *SEPSIS
*ANTIGENS
*ENDOTOXINS
Subjects
Details
- Language :
- English
- ISSN :
- 00099104
- Volume :
- 130
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Clinical & Experimental Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 7570750
- Full Text :
- https://doi.org/10.1046/j.1365-2249.2002.01983.x