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Quantification of glucose, xylose, arabinose, furfural, and HMF in corncob hydrolysate by HPLC-PDA–ELSD
- Source :
-
Carbohydrate Research . May2012, Vol. 353, p111-114. 4p. - Publication Year :
- 2012
-
Abstract
- Abstract: Lignocellulose and other carbohydrates are being studied extensively as potential renewable carbon sources for liquid biofuels and other valuable chemicals. In the present study, a simple, sensitive, selective, and reliable HPLC method using a photodiode array (PDA) detector and an evaporative light scattering detector (ELSD) was developed for the simultaneous determination of important sugars (d(+)-cellobiose, glucose, xylose, and arabinose), furfural and 5-hydroxymethylfurfural (5-HMF) in lignocellulose hydrolysate. The analysis was carried out on an Aminex HPX-87H column (250mm×4.6mm, 5μm particle size). Ultra-pure water with 0.00035M H2SO4 was used as the mobile phase with a flow rate of 0.6mL/min. The temperature of the ELSD drift tube was kept at 50°C, the carrier gas pressure was 350kPa, and the gain was set at 7. Furfural and 5-HMF were quantified on a PDA detector at 275nm and 284nm, respectively. The sugar concentrations were determined by ELSD. This method was validated for accuracy and precision. The regression equation revealed a good linear relationship (r 2 =0.9986±0.0012) within the test ranges. The method showed good reproducibility for the quantification of six analytes in corncob hydrolysate, with intra- and inter-day variations less than 1.12%. This method is also convenient because it allows the rapid analysis of the primary products of biomass hydrolysis and carbohydrate degradation. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 00086215
- Volume :
- 353
- Database :
- Academic Search Index
- Journal :
- Carbohydrate Research
- Publication Type :
- Academic Journal
- Accession number :
- 75183585
- Full Text :
- https://doi.org/10.1016/j.carres.2012.03.029