Back to Search
Start Over
Membrane-displayed peptide ligand activates the pheromone response pathway in Saccharomyces cerevisiae.
- Source :
-
Journal of Biochemistry . May2012, Vol. 151 Issue 5, p551-557. 7p. - Publication Year :
- 2012
-
Abstract
- The budding yeast, Saccharomyces cerevisiae, is an attractive host for studying G protein-coupled receptors (GPCRs). We developed a system in which a peptide ligand specific for GPCR is displayed on yeast plasma membrane. The model system described here is based on yeast plasma membrane display of an analogue of α-factor, which is a peptide ligand for Ste2p, the GPCR that activates the yeast pheromone response pathway. α-Factor analogues, containing linkers of varying lengths and produced in yeast cells, became attached to the cell plasma membrane by linking to the glycosylphosphatidylinositol (GPI)-anchored plasma membrane protein Yps1p. We were able to demonstrate that an optimized α-factor analogue activated the pheromone response pathway in S. cerevisiae, as assessed by a fluorescent reporter assay. Furthermore, it was shown that linker length strongly influenced signalling pathway activation. To our knowledge, this is the first report documenting functional signalling by a plasma membrane-displayed ligand in S. cerevisiae. [ABSTRACT FROM PUBLISHER]
Details
- Language :
- English
- ISSN :
- 0021924X
- Volume :
- 151
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Journal of Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 75054815
- Full Text :
- https://doi.org/10.1093/jb/mvs027