An Efficient in vitro Leaf-based Regeneration and Evaluation of Genetic Fidelity Using ISSR Markers in Woodfordia fruticosa (L.) Kurz.

An efficient in vitro leaf regeneration protocol has been established in Woodfordia fruticosa L. (Lathyraceae), a threatened woody shrub. The leaf segments derived from in vitro-grown plants were cultured on Murashige and Skoog's medium supplemented with Thidiazuron (2.27, 4.54, 6.81, and 9.08 μM) and 6-Benzyladenine (4.4, 8.90, 13.30, and 17.70 μM) alone or in combination with Indole-3-acetic acid (1.14 and 2.28 μM). Maximum number of shoots (15.60 ± 1.15) with highest shoot length (2.90 ± 0.24) were regenerated directly from the leaf explants with a combination of TDZ (4.54 μM) and IAA (2.28 μM), whereas the intervening callus phase was observed in the media supplemented with TDZ or BA alone. The in vitro-regenerated shoots were rooted (100%) on half-strength MS salts fortified with 4.90 μM IBA. The regenerated plantlets hardened in the greenhouse showed an 85% survival rate. ISSR PCR of in vitro-regenerated plantlets reveals their genetic fidelity with the mother plant. [ABSTRACT FROM PUBLISHER]