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Stromal-epithelial interactions modulate the effect of ovarian steroids on goat uterine epithelial cell interleukin-18 release
- Source :
-
Domestic Animal Endocrinology . May2012, Vol. 42 Issue 4, p210-219. 10p. - Publication Year :
- 2012
-
Abstract
- Abstract: A primary role of epithelial-stromal interactions in mediating steroid hormone action in the uterus has been established. The present study was undertaken to determine the mode of ovarian steroid action in regulating IL-18 release by goat endometrial epithelial cells (EECs) in the presence and absence of endometrial stromal cells (ESCs). Primary and telomerase-immortalized goat EECs grown alone or cocultured with ESCs were treated with two ovarian steroids, 17β-estradiol (E2) and progesterone (P4). The IL-18 mRNA and protein expression in EECs were studied by reverse transcript (RT) PCR, ELISA, and Western blot assay. The E2 and/or P4 treatment of EECs led to a significant increase in both IL-18 mRNA and protein expression either in the primary or in the immortalized EECs compared with that in EECs without the steroid treatment. However, in the presence of ESCs, IL-18 expression by EECs treated with steroids was significantly decreased compared with cells untreated with E2 and/or P4. In addition, significantly high abundance of IL-18 mRNA and protein expression by primary and telomerase-immortalized goat EECs was observed in the presence of ESCs compared with those cells without ESCs. These findings suggest that steroids are important for the control of IL-18 expression in goat EECs. Underlying ESCs are needed to mediate the inhibitory effects of steroids on the IL-18 secretory activity of goat EECs in vitro. The IL-18 abundance expressed by goat EECs in vitro are enhanced by underlying ESCs without the treatment of E2 and/or P4. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 07397240
- Volume :
- 42
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Domestic Animal Endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 74308363
- Full Text :
- https://doi.org/10.1016/j.domaniend.2011.12.004