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Garlic Extract Diallyl Sulfide (DAS) Activates Nuclear Receptor CAR to Induce the Sult1e1 Gene in Mouse Liver.

Authors :
Sueyoshi, Tatsuya
Green, William D.
Vinal, Kellie
Woodrum, Tyler S.
Moore, Rick
Negishi, Masahiko
Source :
PLoS ONE. 2011, Vol. 6 Issue 6, p1-7. 7p.
Publication Year :
2011

Abstract

Constituent chemicals in garlic extract are known to induce phase I and phase II enzymes in rodent livers. Here we have utilized Car+/+ and Car2/2 mice to demonstrate that the nuclear xenobiotic receptor CAR regulated the induction of the estrogen sulfotransferase Sult1e1 gene by diallyl sulfide (DAS) treatment in mouse liver. DAS treatment caused CAR accumulation in the nucleus, resulting in a remarkable increase of SULT1E1 mRNA (3,200 fold) and protein in the livers of Car+/+ females but not of Car2/2 female mice. DAS also induced other CAR-regulated genes such as Cyp2b10, Cyp3a11 and Gadd45b. Compared with the rapid increase of these mRNA levels, which began as early as 6 hourrs after DAS treatment, the levels of SULT1E1 mRNA began increasing after 24 hours. This slow response to DAS suggested that CAR required an additional factor to activate the Sult1e1 gene or that this activation was indirect. Despite the remarkable induction of SULT1E1, there was no decrease in the serum levels of endogenous E2 or increase of estrone sulfate while the clearance of exogenously administrated E2 was accelerated in DAS treated mice. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
19326203
Volume :
6
Issue :
6
Database :
Academic Search Index
Journal :
PLoS ONE
Publication Type :
Academic Journal
Accession number :
74275223
Full Text :
https://doi.org/10.1371/journal.pone.0021229