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Molecular and phenotypic characterization of Acinetobacter strains able to degrade diesel fuel

Authors :
Mara, Kostlend
Decorosi, Francesca
Viti, Carlo
Giovannetti, Luciana
Papaleo, Maria Cristiana
Maida, Isabel
Perrin, Elena
Fondi, Marco
Vaneechoutte, Mario
Nemec, Alexandr
van den Barselaar, Maria
Dijkshoorn, Lenie
Fani, Renato
Source :
Research in Microbiology. Apr2012, Vol. 163 Issue 3, p161-172. 12p.
Publication Year :
2012

Abstract

Abstract: Characterization of bacterial communities in oil-contaminated soils and evaluation of their degradation capacities may serve as a guide for improving remediation of such environments. Using physiological and molecular methods, the aim of this work was to characterize 17 Acinetobacter strains (13 species) able to use diesel fuel oil as sole carbon and energy source. The strains were first tested for their ability to grow on different alkanes on minimal medium containing high NaCl concentrations. The envelope hydrophobicity of each strain was assessed by microbial adhesion to the hydrocarbon test (MATH) when grown in LB medium or minimal medium containing succinate or diesel fuel. Most strains were hydrophobic both in LB and minimal medium, except for strain Acinetobacter venetianus VE-C3 that was hydrophobic only in minimal medium. Furthermore, two A. venetianus strains, RAG-1T and LUH 7437, and strain ATCC 17905 (genomic species 13BJ) displayed biosurfactant activity. The alkM gene encoding alkane hydroxylase was detected in the chromosome of the 15 strains by PCR amplification, sequencing and Southern blot analysis. Phenotype microarray analysis performed on the five A. venetianus strains revealed that they differentially used purines as N-source and confirmed that they are unable to use carbohydrates. [Copyright &y& Elsevier]

Details

Language :
English
ISSN :
09232508
Volume :
163
Issue :
3
Database :
Academic Search Index
Journal :
Research in Microbiology
Publication Type :
Academic Journal
Accession number :
74096514
Full Text :
https://doi.org/10.1016/j.resmic.2011.12.002