Back to Search
Start Over
Bovine lactoferrin induces interleukin-11 production in a hepatitis mouse model and human intestinal myofibroblasts.
- Source :
-
European Journal of Nutrition . Apr2012, Vol. 51 Issue 3, p343-351. 9p. 1 Black and White Photograph, 3 Graphs. - Publication Year :
- 2012
-
Abstract
- Purpose: Orally administered bovine lactoferrin (bLF) exerts an anti-inflammatory effect on hepatitis and colitis animal models. To investigate the mechanism underlying the action of bLF, we explored the expression of inflammation-related factors in the intestine of a hepatitis mouse model after the oral administration of bLF and in several human intestinal cell lines treated with bLF. Methods: The effects of bLF on the expression of interleukin-11 (IL-11) and bone morphogenetic protein 2 (BMP2) in the intestinal mucosa of a hepatitis mouse model as well as in cell cultures of human intestinal epithelial cells, myofibroblasts, and monocytes were examined using the real-time reverse transcription polymerase chain reaction. Epithelial cells and myofibroblasts were also cocultured using transwells. bLF transport, and IL-11 and BMP2 induction, as well as the interactions between the two cell types, were then analyzed after bLF treatment. Results: In vivo, oral bLF administration increased the production of IL-11 and BMP2 in intestinal specimens. In vitro, bLF only stimulated the production of IL-11 in human intestinal myofibroblasts; i.e., it had no effect on BMP2 production in any cell type. In the transwell cocultures, bLF passed through the epithelium and directly stimulated IL-11 production in the myofibroblasts on the basolateral side. The IL-11 produced in the myofibroblasts subsequently acted protectively on the epithelial cells of the coculture. Conclusions: bLF upregulated the activity of anti-inflammatory factors, such as IL-11, in the intestine of a hepatitis mouse model and human intestinal myofibroblasts. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ANIMAL experimentation
*ANTI-inflammatory agents
*BIOLOGICAL models
*BIOPHYSICS
*BONE morphogenetic proteins
*EPITHELIUM
*FIBROBLASTS
*IMMUNOBLOTTING
*INTERLEUKINS
*INTESTINAL mucosa
*INTESTINES
*RESEARCH methodology
*MICE
*MILK proteins
*MONOCYTES
*ORAL drug administration
*POLYMERASE chain reaction
*STATISTICS
*T-test (Statistics)
*DATA analysis
*REVERSE transcriptase polymerase chain reaction
*DATA analysis software
*DESCRIPTIVE statistics
*PHARMACODYNAMICS
Subjects
Details
- Language :
- English
- ISSN :
- 14366207
- Volume :
- 51
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- European Journal of Nutrition
- Publication Type :
- Academic Journal
- Accession number :
- 73823390
- Full Text :
- https://doi.org/10.1007/s00394-011-0219-y