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Neurotrophin-3 Gene-Modified Schwann Cells Promote TrkC Gene-Modified Mesenchymal Stem Cells to Differentiate into Neuron-Like Cells in Poly(Lactic-Acid-Co-Glycolic Acid) Multiple-Channel Conduit.
- Source :
-
Cells Tissues Organs . 2012, Vol. 195 Issue 4, p313-322. 10p. 5 Color Photographs. - Publication Year :
- 2012
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Abstract
- Rapid progress in the field of nerve tissue engineering has opened up the way for new therapeutic strategies for spinal cord injury (SCI). Bone marrow-derived mesenchymal stem cells (MSCs) could be differentiated into neural lineages, which can be used as a potential cell source for nerve repair. Schwann cells (SCs) have been reported to support structural and functional recovery of SCI. In this study, we co-cultured neurotrophin-3 (NT-3) gene-modified SCs and NT-3 receptor tyrosine protein kinase C (TrkC) gene-modified MSCs in a three-dimensional porous poly(lactic-acid-co-glycolic acid) (PLGA) conduit with multiple channels in vitro for 14 days. Our results showed that more than 50% of the grafted MSCs were MAP2- and β-III-tubulin-positive cells, and the MSCs expressed a high level of β-III-tubulin detected by Western blotting, indicating a high rate of neuronal differentiation. Furthermore, immunostaining of PSD95 revealed the formation of a synapse-like structure, which was confirmed under electron microscopy. In conclusion, co-culture of NT-3 gene-modified SCs and TrkC gene-modified MSCs in the PLGA multiple-channeled conduit can promote MSCs' differentiation into neuron-like cells with synaptogenesis potential. Our study provides a biological basis for future application of this artificial MSCs/SCs/PLGA complex in the SCI treatment. Copyright © 2011 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 14226405
- Volume :
- 195
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Cells Tissues Organs
- Publication Type :
- Academic Journal
- Accession number :
- 73522811
- Full Text :
- https://doi.org/10.1159/000327724