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Cellular responses to Schistosoma japonicum cathepsin D aspartic protease.
- Source :
-
Parasite Immunology . Jul2002, Vol. 24 Issue 7, p363-367. 5p. - Publication Year :
- 2002
-
Abstract
- Summary Lymphocyte proliferation and cytokine production were measured in groups of mice vaccinated (but not subsequently challenge infected) with recombinant forms of Schistosoma japonicum cathepsin D aspartic protease, rSjASP1 (expressed in bacteria; enzymatically inactive) and rSjASP2 (expressed in insect cells; enzymatically active). Both forms of the schistosome enzyme induced significant proliferation of splenocytes recovered from vaccinated mice, and expression of interferon (IFN)-γ, interleukin (IL)-4 and IL-10 mRNA in these cells was detected using reverse transcriptase-polymerase chain reaction. Secretion of IFN-γ, IL-4 and IL-10 by splenocytes from vaccinated mice was confirmed and quantified using enzyme-linked immunosorbent assay. IFN-γ was the most abundant cytokine produced, followed by IL-4 and IL-10 in rank order. These findings indicated that vaccination of mice with the schistosome protease induces a mixed Th1/Th2 cytokine response, which may explain the modest level of protection after challenge infection in cathepsin d-vaccinated mice, reported previously. [ABSTRACT FROM AUTHOR]
- Subjects :
- *SCHISTOSOMA japonicum
*PROTEOLYTIC enzymes
*CYTOKINES
Subjects
Details
- Language :
- English
- ISSN :
- 01419838
- Volume :
- 24
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- Parasite Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 7114482
- Full Text :
- https://doi.org/10.1046/j.1365-3024.2002.00475.x