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Effect of GDF-5 and BMP-2 on the expression of tendo/ligamentogenesis-related markers in human PDL-derived cells.
- Source :
-
Oral Diseases . Mar2012, Vol. 18 Issue 2, p206-212. 7p. 2 Black and White Photographs, 1 Graph. - Publication Year :
- 2012
-
Abstract
- Oral Diseases (2012) 18, 206-212 Objectives: The effect of growth differentiation factor 5 and bone morphogenetic protein 2 on human periodontal ligament-derived cells was investigated with special reference to tendo/ligamentogenesis-related markers. Materials and Methods: Effects of each factor were analyzed by quantitative PCR for scleraxis and tenomodulin and by western blotting for scleraxis. After exposure to those factors, STRO-1-positive and STRO-1-negative fractions of human periodontal ligament tissues were isolated with an immunomagnetic cell sorting system, and the expression of scleraxis in each fraction was analyzed by western blotting. Non-separated crude cells were used as a control. Results: Growth differentiation factor 5 and bone morphogenetic protein 2 did not increase alkaline phosphatase activity in crude periodontal ligament-derived cells. Growth differentiation factor 5, but not bone morphogenetic protein 2, increased the expression of scleraxis in crude, STRO-1-positive and STRO-1-negative periodontal ligament-derived cells. The expression of scleraxis in STRO-1-positive periodontal ligament-derived cells was significantly less compared to that in crude P2 and STRO-1-negative periodontal ligament-derived cells. Conclusion: Growth differentiation factor 5 induced the expression of scleraxis and may enhance tendo/ligamentogenesis in human periodontal ligament-derived cells. The expression of scleraxis was higher in STRO-1-negative fraction, suggesting more differentiated state of the cells. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 1354523X
- Volume :
- 18
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Oral Diseases
- Publication Type :
- Academic Journal
- Accession number :
- 70331251
- Full Text :
- https://doi.org/10.1111/j.1601-0825.2011.01871.x