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Deferoxamine pretreatment prevents Cr(VI)-induced nephrotoxicity and oxidant stress: Role of Cr(VI) chelation

Authors :
Molina-Jijón, Eduardo
Zarco-Márquez, Guillermo
Medina-Campos, Omar Noel
Zataraín-Barrón, Zyanya Lucía
Hernández-Pando, Rogelio
Pinzón, Enrique
Zavaleta, Rosa Marina
Tapia, Edilia
Pedraza-Chaverri, José
Source :
Toxicology. Jan2012, Vol. 291 Issue 1-3, p93-101. 9p.
Publication Year :
2012

Abstract

Abstract: Deferoxamine (DFO) is a recognized iron chelator which has been shown to exert nephroprotection in models of toxic nephropathies. In the present work the potential protective effects of DFO against Cr(VI)-induced nephrotoxicity and oxidant stress were evaluated. Rats were injected with a single injection (15mg/kg, s.c.) of potassium dichromate (K2Cr2O7). DFO was given as a single i.p. injection 30min before K2Cr2O7 administration at three different doses (100, 200 and 400mg/kg). It was found that DFO pretreatment attenuated, in a dose-dependent way, K2Cr2O7-induced renal dysfunction and structural alterations evaluated by serum creatinine, blood urea nitrogen, creatinine clearance, proteinuria, plasma glutathione peroxidase activity, urinary excretion of N-acetyl-β-d-glucosaminidase and histological analyses. Furthermore, DFO prevented the K2Cr2O7-induced renal oxidant stress and the decrease in the activity of the antioxidant enzymes superoxide dismutase, glutathione reductase, glutathione peroxidase, glutathione-S-transferase and catalase. Finally it was found that DFO, at 400mg/kg, decreases renal Cr(VI) content which prompted us to evaluate the potential Cr(VI) chelating properties of this compound. Indeed was found in an in vitro assay that DFO was an effective Cr(VI) chelator with an IC50 of 800μg. In additional groups of rats was found that DFO posttreatment was ineffective to attenuate K2Cr2O7-induced nephrotoxicity and renal oxidant stress. Furthermore, DFO was unable to modify urinary excretion of total chromium. The nephroprotective effect of DFO against Cr(VI)-induced nephrotoxicity and oxidant stress may be explained, at least partially, by the ability of DFO to chelate Cr(VI) and to attenuate renal Cr(VI) content. However, it cannot be excluded that the ability of DFO to chelate iron may also be involved in the protection observed in our study. [Copyright &y& Elsevier]

Details

Language :
English
ISSN :
0300483X
Volume :
291
Issue :
1-3
Database :
Academic Search Index
Journal :
Toxicology
Publication Type :
Academic Journal
Accession number :
70154839
Full Text :
https://doi.org/10.1016/j.tox.2011.11.003