Towards a rapid molecular diagnostic for melioidosis: Comparison of DNA extraction methods from clinical specimens

Abstract: Optimising DNA extraction from clinical samples for Burkholderia pseudomallei Type III secretion system real-time PCR in suspected melioidosis patients confirmed that urine and sputum are useful diagnostic samples. Direct testing on blood remains problematic; testing DNA extracted from plasma was superior to DNA from whole blood or buffy coat. [Copyright &y& Elsevier]