Effect of light intensity on Opisthorchis viverrini cercarial shedding levels from Bithynia snails — A preliminary study

Abstract: Opisthorchis viverrini requires Bithynia snails as the first intermediate host and cyprinid fish as the second intermediate host. Very low natural infection rates have been reported in Bithynia snails, but very high rates have been found in cyprinid fish in the same endemic region. This study investigated the effect of light intensity, the most important stimulus, on the quantity of O. viverrini cercariae shed from naturally infected Bithynia (Digoniostoma) siamensis goniomphalos snails. Snails were evaluated for cercariae output every hour after exposure to various light intensities for a total period of 7h. The same infected snail was tested under different intensities of light: in the dark, and at 1000, 3000 and 5000lx. The data showed that under exposure to 1000 and 3000lx of light, the average percentage and number of cercariae released were higher than that exposed to 5000lx during the first 2h of the experiment. In contrast, under higher illumination (5000lx) a longer time (6h) was required to stimulate the peak emergence of cercariae. Darkness was not able to induce O. viverrini cercariae emergence. Among the three intensities of light, exposure at 1000lx induced the highest average number of released cercariae per snail and the highest percentage of cercarial emergence within the first 2h (125, 54.86%), followed by exposure at 3000lx (69, 25.58%) and 5000lx (12, 7.78%). The results suggest that the light intensity of 1000lx for 2h would be optimal for O. viverrini cercarial shedding from naturally infected B. (D.) siamensis goniomphalos snails. [Copyright &y& Elsevier]