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Selective immobilization of proteins to self-assembled monolayers presenting active site-directed capture ligands.

Authors :
Hodneland, Christian D.
Young-Sam Lee
Dal-Hee Min
Mrksich, Milan
Source :
Proceedings of the National Academy of Sciences of the United States of America. 4/16/2002, Vol. 99 Issue 8, p5048. 5p. 4 Diagrams, 9 Graphs.
Publication Year :
2002

Abstract

Describes a method for the selective immobilization of proteins to self-assembled monolayers in active site-directed capture ligands. Construction of cutinase-calmodulin fusion protein; Purification of proteins; Development of protein microarrays.

Details

Language :
English
ISSN :
00278424
Volume :
99
Issue :
8
Database :
Academic Search Index
Journal :
Proceedings of the National Academy of Sciences of the United States of America
Publication Type :
Academic Journal
Accession number :
6839092
Full Text :
https://doi.org/10.1073/pnas.072685299