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β-Galactosidase-Based Colorimetric Paper Sensor for Determination of Heavy Metals.

Authors :
Hossain, S. M. Zakir
Brennan, John D.
Source :
Analytical Chemistry. 11/15/2011, Vol. 83 Issue 22, p8772-8778. 7p.
Publication Year :
2011

Abstract

We demonstrate a novel approach for rapid, selective, and sensitive detection of heavy metals using a solid-phase bioactive lab-on-paper sensor that is inkjet printed with sol-gel entrapped reagents to allow colorimetric visualization of the enzymatic activity of ß-galactosidase (B-GAL). The bioactive paper assay is able to detect a range of heavy metals, either alone or as mixtures, in as little as 10 min, with detection limits as follows: Hg(II) = 0.001 ppm; Ag(I) = 0.002 ppm, Cu(II) = 0.020 ppm; Cd(II) = 0.020 ppm; Pb(II) = 0.140 ppm; Cr(VI) = 0.150 ppm; Ni(II) = 0.230 ppm. The paper-based assay was immune to interferences from nontoxic metal ions such as Na+ or K+, could be used to detect heavy metals that were spiked into tap water or lake water, and provided quantitative data that was in agreement with values obtained by atomic absorption. With the incorporation of standard chromogenic metal sensing reagents into a multiplexed bioactive paper sensor, it was possible to identify specific metals in mixtures, albeit with much lower detection limits than were obtained with the enzymatic assay. The paper-based sensor should be valuable for rapid, on-site screening of trace levels of heavy metals in resource limited areas and developing countries. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00032700
Volume :
83
Issue :
22
Database :
Academic Search Index
Journal :
Analytical Chemistry
Publication Type :
Academic Journal
Accession number :
67653520
Full Text :
https://doi.org/10.1021/ac202290d