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A Protein Tyrosine Kinase in the Interferon α/β Signaling Pathway.
- Source :
-
Journal of Immunology . 12/1/2011, Vol. 187 Issue 11, p5479-5488. 10p. - Publication Year :
- 2011
-
Abstract
- The mutant human cell line 11,1 is unresponsive to interferon a. Here we describe the genetic complementatlon of this mutant and the Identification and cloning of the wild-type gene that corrects the defect. Using transtection with genomic DNA in conjunction with a powerful back-selection, we isolated a cosmid that reveils the mutant phenotype of 11,1 cells. The cosmid encodes a single message whose level is greatly reduced in mutant cells. Complementary DNA5 were cloned and found to be virtually Identical to tyk2, a humafl mRNA encoding a non-receptor protein tyrosine kinase of previously unknown function. This finding shows that fyk2 links the interferon α/β receptor to the cytoplasmic transcription factor that mediates activation of interferon-responsive genes. [ABSTRACT FROM AUTHOR]
- Subjects :
- *PROTEIN-tyrosine kinases
*INTERFERONS
*HUMAN cloning
*CELL lines
*CYTOPLASM
Subjects
Details
- Language :
- English
- ISSN :
- 00221767
- Volume :
- 187
- Issue :
- 11
- Database :
- Academic Search Index
- Journal :
- Journal of Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 67635111