Capillary zone electrophoresis as a potential technique for the simultaneous determination of sulfadoxine and pyrimethamine in tablet formulations

Abstract: A novel, simple and rapid capillary zone electrophoresis method with UV detection has been developed for the simultaneous determination of pyrimethamine and sulfadoxine in tablet formulations. The compounds are separated in 6min in a fused silica capillary, 30cm long (20cm to detector)×50μm using a 100mM phosphate buffer pH 7.2 as background electrolyte, a 330Vcm−1 electric field and a detection wavelength of 214nm. Analysis of different tablet formulations has shown a good agreement with the liquid chromatography method described in the United States Pharmacopoeia. Main advantages of the CZE method are the rapid set-up of instrumentation and capillary equilibration, short analysis time and low running cost. [Copyright &y& Elsevier]