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Quantitative MALDI-TOF-MS Using Stable-isotope Labeling: Application to the Analysis of N-glycans of Recombinant α-1 Antitrypsin Produced Using Different Culture Parameters.
- Source :
-
Journal of Carbohydrate Chemistry . May-Aug2011, Vol. 30 Issue 4-6, p320-333. 14p. - Publication Year :
- 2011
-
Abstract
- It is usually difficult to compare quantitatively different sets of glycan samples that have slight differences among themselves using MALDI-TOF-MS. To overcome this problem, 13C-labeled glycans were used as internal standards during the measurement of the N-glycan samples permethylated with 12C-methyl iodide. This method was used to analyze the N-glycans from human α-1 antitrypsin (A1AT) that was recombinantly produced using various culture parameters. Several pH values, dissolved oxygen concentrations, and temperatures were used to produce A1AT using a batch process system, and we investigated the extent to which the glycosylation pattern of A1AT was influenced by these parameters. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 07328303
- Volume :
- 30
- Issue :
- 4-6
- Database :
- Academic Search Index
- Journal :
- Journal of Carbohydrate Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 67129576
- Full Text :
- https://doi.org/10.1080/07328303.2011.605194