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In Vitro and In Vivo Evaluation of a Folate-Targeted Copolymeric Submicrohydrogel Based on N-Isopropylacrylamide as 5-Fluorouracil Delivery System.
- Source :
-
Polymers (20734360) . Sep2011, Vol. 3 Issue 3, p1107-1125. 19p. 1 Color Photograph, 1 Black and White Photograph, 2 Charts, 5 Graphs. - Publication Year :
- 2011
-
Abstract
- Folate-targeted poly[(p-nitrophenyl acrylate)-co-(N-isopropylacrylamide)] nanohydrogel (F-SubMG) was loaded with 5-fluorouracil (5-FU) to obtain low (16.3 ± 1.9 μg 5-FU/mg F-SubMG) and high (46.8 ± 3.8 μg 5-FU/mg F-SubMG) load 5-FU-loaded F-SubMGs. The complete in vitro drug release took place in 8 h. The cytotoxicity of unloaded F-SubMGs in MCF7 and HeLa cells was low; although it increased for high F-SubMG concentration. The administration of 10 μM 5-FU by 5-FU-loaded F-SubMGs was effective on both cellular types. Cell uptake of F-SubMGs took place in both cell types, but it was higher in HeLa cells because they are folate receptor positive. After subcutaneous administration (28 mg 5-FU/kg b.w.) in Wistar rats, F-SubMGs were detected at the site of injection under the skin. Histological studies indicated that the F-SubMGs were surrounded by connective tissue, without any signs of rejections, even 60 days after injection. Pharmacokinetic study showed an increase in MRT (mean residence time) of 5-FU when the drug was administered by drug-loaded F-SubMGs. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 20734360
- Volume :
- 3
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Polymers (20734360)
- Publication Type :
- Academic Journal
- Accession number :
- 67028574
- Full Text :
- https://doi.org/10.3390/polym3031107