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Frequency-domain phase fluorometry in the presence of dark states: A numerical study
- Source :
-
Chemical Physics Letters . Nov2011, Vol. 516 Issue 1-3, p40-44. 5p. - Publication Year :
- 2011
-
Abstract
- Abstract: Fluorescence anomalous phase advance (FAPA) is a newly discovered spectroscopy phenomenon: instead of lagging behind the modulated light, fluorescence signal can exhibit FAPA as if it precedes the excitation source in time. While FAPA offers a promising technique for probing dark state lifetime, the underlying mechanism is not fully elucidated. Herein we investigate frequency-domain phase fluorometry as a result of intricate interplay between a short-lived fluorescent state and a long-lived dark state. In particular, the quantitative dependence on modulation frequency, excitation intensity, nonradiative decay, intersystem crossing and dark-state lifetime are explored respectively. A comprehensive view of phase fluorometry emerges consequently. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 00092614
- Volume :
- 516
- Issue :
- 1-3
- Database :
- Academic Search Index
- Journal :
- Chemical Physics Letters
- Publication Type :
- Academic Journal
- Accession number :
- 66946076
- Full Text :
- https://doi.org/10.1016/j.cplett.2011.09.054