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Reversible Fluorescent Probe for Highly Selective and Sensitive Detection of Mercapto Biomolecules.
- Source :
-
Inorganic Chemistry . 7/18/2011, Vol. 50 Issue 14, p6543-6551. 9p. - Publication Year :
- 2011
-
Abstract
- A coumarin-derived complex, Hg2L2, was reported as a highly sensitive and selective probe for the detection of mercapto biomolecules in aqueous solution. The addition of Cys to a 99% aqueous solution of Hg2L2 resulted in rapid and remarkable fluorescence OFF--ON (emission at 525 nm) due to the ligand-exchange reaction of Cys with L coordinated to Hg2+. The increased fluorescence can be completely quenched by Hg2+ and recovered again by the subsequent addition of Cys. Such a fluorescence OFF--ON circle can be repeated at least 10 times by the alterative addition of Cys and Hg2+ to the solution of Hg2L2, indicating that it can be used as a convertible and reversible probe for the detection of Cys. The interconversion of Hg2L2 and L via the decomplexation/complexation by the modulation of Cys/Hg2+ was definitely verified from their crystal structures. Other competitive amino acids without a thiol group cannot induce any fluorescence changes, implying that Hg2L2 can selectively determine mercapto biomolecules. Using confocal fluorescence imaging, L/Hg2L2 as a pair of reversible probes can be further applied to track and monitor the self-detoxification process of Hg2+ ions in SYS5 cells. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00201669
- Volume :
- 50
- Issue :
- 14
- Database :
- Academic Search Index
- Journal :
- Inorganic Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 66804942
- Full Text :
- https://doi.org/10.1021/ic200181p