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A Turn-on Fluorescent Iron Complex and Its Cellular Uptake.
- Source :
-
Inorganic Chemistry . 9/19/2011, Vol. 50 Issue 18, p9178-9183. 6p. - Publication Year :
- 2011
-
Abstract
- In the treatment of chronic iron overload disorders, ligands capable of complexing so-called "labile" (nonprotein bound) Fe are required to enter iron-loaded cells, sequester excess Fe, and then exit the cell (and the body) as an intact Fe complex. Despite the emergence of several ligand families that show high activity in mobilizing intracellular Fe, the mechanism and the locations of these subcellular labile Fe pools are still poorly understood. Our previous studies have unearthed a class of heterocyclic hydrazine-based chelators (e.g., benzoyl picolinoyl hydrazine, H2BPH) that show excellent activity at mobilizing Fe from Fe-loaded cells. Herein, we have grafted a fluorescent tag (rhodamine B) onto H2BPH to generate a ligand (L1) that is nonfluorescent in its uncomplexed form but becomes strongly fluorescent in complex with FeIII. The free ligand and its 1:2 Fe complex [FeIII(L1)2]3+ have both been fully characterized spectroscopically and with X-ray crystallography. Confocal fluorescent microscopy of HeLa cells incubated with [FeIII(L1)2]3+ shows that the complex rapidly enters HeLa cells and localizes within endosomes/lysosomes. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00201669
- Volume :
- 50
- Issue :
- 18
- Database :
- Academic Search Index
- Journal :
- Inorganic Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 66701221
- Full Text :
- https://doi.org/10.1021/ic201495r