THIRTEEN MICROSATELLITES DEVELOPED BY SSR-ENRICHED PYROSEQUENCING FOR SOLANUM ROSTRATUM (SOLANACEAE) AND RELATED SPECIES.

• Premise of the study: Microsatellite markers were developed using second-generation sequencing in Solanum rostratum as a tool to study the reproductive biology and genetic structure of this invasive species. • Methods and Results: Thirteen microsatellites were successfully discovered and amplified in a single multiplexed PCR. All loci showed genetic variation in S. rostratum . Cross-amplification in five closely related taxa was successful for a subset of loci. • Conclusions: The set of 13 microsatellite markers developed here provides a time-effective and cost-effective genetic tool to study the reproductive biology of S. rostratum . The demonstrated transferability of the PCR multiplex to related taxa also highlights its usefulness for evolutionary studies across Solanum sect. Androceras. [ABSTRACT FROM AUTHOR]