Zn[sup 2+] Binding Properties of Single-Point Mutants of the C-Terminal Zinc Finger of the HIV-1 Nucleocapsid Protein: Evidence of a Critical Role of Cysteine 49 in Zn[sup2+] Dissociation.

Authors :: Bombarda, E.
Cherradi, H.
Morellet, N.
Roques, B.P.
Mély, Y.
Source :: Biochemistry. 4/2/2002, Vol. 41 Issue 13, p4312. 9p. 1 Diagram, 4 Charts, 4 Graphs.
Publication Year :: 2002
Abstract: Analyzes the Zinc[sup 2+] binding properties of single-point mutants of the C-terminal zinc finger of the HIV-1 nucleocapsid protein. Evidence of a critical role of cysteine 49 in Zinc[sup 2+] dissociation; Details on the spectroscopic properties of Cobalt[sup 2+]; Use of fluorescence of Trp37 to monitor the binding of Zinc[sup 2+].

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