Back to Search Start Over

Human oocytes reprogram somatic cells to a pluripotent state.

Authors :
Noggle, Scott
Fung, Ho-Lim
Gore, Athurva
Martinez, Hector
Satriani, Kathleen Crumm
Prosser, Robert
Oum, Kiboong
Paull, Daniel
Druckenmiller, Sarah
Freeby, Matthew
Greenberg, Ellen
Zhang, Kun
Goland, Robin
Sauer, Mark V.
Leibel, Rudolph L.
Egli, Dieter
Source :
Nature. 10/6/2011, Vol. 478 Issue 7367, p70-75. 6p. 1 Diagram, 3 Graphs.
Publication Year :
2011

Abstract

The exchange of the oocyte's genome with the genome of a somatic cell, followed by the derivation of pluripotent stem cells, could enable the generation of specific cells affected in degenerative human diseases. Such cells, carrying the patient's genome, might be useful for cell replacement. Here we report that the development of human oocytes after genome exchange arrests at late cleavage stages in association with transcriptional abnormalities. In contrast, if the oocyte genome is not removed and the somatic cell genome is merely added, the resultant triploid cells develop to the blastocyst stage. Stem cell lines derived from these blastocysts differentiate into cell types of all three germ layers, and a pluripotent gene expression program is established on the genome derived from the somatic cell. This result demonstrates the feasibility of reprogramming human cells using oocytes and identifies removal of the oocyte genome as the primary cause of developmental failure after genome exchange. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00280836
Volume :
478
Issue :
7367
Database :
Academic Search Index
Journal :
Nature
Publication Type :
Academic Journal
Accession number :
66357998
Full Text :
https://doi.org/10.1038/nature10397