1,3,5,7-Tetramethyl-8-( N-hydroxysuccinimidyl butyric ester)difluoroboradiaza- s-indacene as a new fluorescent labeling reagent for HPLC determination of amino acid neurotransmitters in the cerebral cortex of mice.

1,3,5,7-Tetramethyl-8-( N-hydroxysuccinimidyl butyric ester)difluoroboradiaza- s-indacene (TMBB-Su), a new BODIPY-based fluorescent probe, was designed and synthesized for the labeling of amino compounds. It was used as a pre-column derivatizing reagent for determination of amino acid neurotransmitters by high-performance liquid chromatography (HPLC). The fluorescence quantum yield in acetonitrile increased from 0.84 to 0.95 when it reacted with amino acid neurotransmitters. Derivatization of TMBB-Su with seven amino acid neurotransmitters was completed within 30 min at 25 °C in 24.0 mmol L pH 7.8 boric acid buffer. The separation was performed on a C column with methanol-water-buffer 55:35:10 ( v/ v) as mobile phase (buffer: 0.10 mol L HCit-0.10 mol L NaOH). Interference from the other concomitant amino acids was eliminated successfully by means of pH gradient elution. With fluorescence detection at 494 and 504 nm for excitation and emission, respectively, the limits of detection (signal-to-noise ratio = 3) were from 2.1 to 12.0 nmol L. The proposed method has been used to determine amino acid neurotransmitters in the cerebral cortex of mice with cerebral ischemia at the convalescence stage with satisfactory recoveries varying from 94.9 to 105.2%. [ABSTRACT FROM AUTHOR]