Back to Search
Start Over
Melatonin inhibits IL-1β-induced monolayer permeability of human umbilical vein endothelial cells via Rac activation.
- Source :
-
Journal of Pineal Research . Sep2011, Vol. 51 Issue 2, p220-225. 6p. - Publication Year :
- 2011
-
Abstract
- Melatonin is a key factor in the coordination of circadian rhythms and seasonal reproduction. Melatonin and its metabolites directly scavenge free radicals, increase the expression of antioxidant enzymes, and play a role in the anti-inflammatory phase of defense responses. At present, there are no direct data available as to melatonin's possible influence on endothelial cell monolayer permeability, which is a major biological process responsible for vascular diseases. The aim of this study was to investigate the effect of melatonin on IL-1β-induced human umbilical vein endothelial cells (HUVECs) monolayer permeability and then to test the involvement of small GTPase Rac in the melatonin-induced endothelial barrier-protective effects as well as cell contact reorganization. It was found that IL-1β treatment increased the permeability of HUVECs monolayer, disrupted adherens junctions, and down-regulated the expression of VE-cadherin which is the main functional protein of adherens junctions. Melatonin, however, decreased dextran permeability and increased intercellular adherens junction areas reflecting an endothelial cell barrier-protective response. Furthermore, melatonin dramatically improved IL-1β-induced Rac inactivation. Our results show that the barrier-protective effects of melatonin on endothelial cells are mediated by Rac activation and leads to enhancement of adherens junctions. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 07423098
- Volume :
- 51
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Journal of Pineal Research
- Publication Type :
- Academic Journal
- Accession number :
- 64376638
- Full Text :
- https://doi.org/10.1111/j.1600-079X.2011.00882.x