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Production of long helical capsid of Nipah virus by Pichia pastoris
- Source :
-
Process Biochemistry . Sep2011, Vol. 46 Issue 9, p1871-1874. 4p. - Publication Year :
- 2011
-
Abstract
- Abstract: The nucleocapsid (N) protein of Nipah virus (NiV) produced in a recombinant host can replace the use of inactivated virus as a diagnostic reagent because it is safer and affordable. The aim of this study was to express the N protein in Pichia pastoris. The N gene of NiV was cloned into the yeast expression vector, pPICZ B and expressed in P. pastoris. The recombinant N protein of NiV was purified using sucrose density gradient ultracentrifugation and was confirmed with Western blotting using rabbit anti-N antibody. The P. pastoris expressed N protein self-assembled into helical structures as large as 1.5μm as shown in an electron micrograph. ELISA analysis performed with the swine sera obtained during the viral outbreak proved that the recombinant N protein to be highly antigenic. The NiV N protein produced in P. pastoris serves as an alternative to the recombinant N protein produced in Escherichia coli. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 13595113
- Volume :
- 46
- Issue :
- 9
- Database :
- Academic Search Index
- Journal :
- Process Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 63982385
- Full Text :
- https://doi.org/10.1016/j.procbio.2011.06.004