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Highly Hydrophilic Surfaces from Polyglycidol Grafts with Dual Antifouling and Specific Protein Recognition Properties.

Authors :
Sarra Gam-Derouich
Monika Gosecka
Sandrine Lepinay
Mireille Turmine
Benjamin Carbonnier
Teresa Basinska
Stanislaw Slomkowski
Marie-Claude Millot
Ali Othmane
Dalila Ben Hassen-Chehimi
Mohamed M. Chehimi
Source :
Langmuir. Aug2011, Vol. 27 Issue 15, p9285-9294. 10p.
Publication Year :
2011

Abstract

Homopolymer grafts from α-tert-butoxy-ω-vinylbenzyl-polyglycidol (PGL) were prepared on gold and stainless steel (SS) substrates modified by 4-benzoyl-phenyl (BP) moieties derived from the electroreduction of the parent salt 4-benzoyl benzene diazonium tetrafluoroborate. The grafted BP aryl groups efficiently served to surface-initiate photopolymerization (SIPP) of PGL. In similar conditions, SIPP of hydroxyethyl methacrylate (HEMA) permitted the production of PHEMA grafts as model surfaces. Water contact angles were found to be 66°, 15°, and 0° for SS-BP, SS-PHEMA, and SS-PPGL, respectively. The spontaneous spreading of water drops on SS-PPGL was invariably observed with 1.5 μL water drops. PPGL thus appears as a superhydrophilic polymer. Resistance to nonspecific adsorption of proteins of PPGL and PHEMA grafts on gold was evaluated by surface plasmon resonance (SPR) using antibovine serum albumin (anti-BSA). The results conclusively show that PPGL-grafts exhibit enhanced resistance to anti-BSA adsorption compared to the well-known hydrophilic PHEMA. PPGL grafts were further modified with BSA through the carbonyldiimidazole activation of the OH groups providing immunosensing surfaces. The so-prepared PPGL-grafted BSA hybrids specifically interacted with anti-BSA in PBS as compared to antimyoglobin. It is clear that the superhydrophilic character of PPGL grafts opens new avenues for biomedical applications where surfaces with dual functionality, namely, specific protein grafting together with resistance to biofouling, are required. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
07437463
Volume :
27
Issue :
15
Database :
Academic Search Index
Journal :
Langmuir
Publication Type :
Academic Journal
Accession number :
63247976
Full Text :
https://doi.org/10.1021/la200290k