Back to Search
Start Over
Potential of confocal microscopes to resolve in the 50–100 nm range.
- Source :
-
Applied Physics Letters . 12/9/1996, Vol. 69 Issue 24, p3644. 3p. 3 Diagrams, 2 Graphs. - Publication Year :
- 1996
-
Abstract
- We determine the resolution of high-performance confocal microscopes by measuring the three-dimensional point–spread function (3D-PSF) of an optimized confocal setup. The 3D-PSF is standardized by recording the scattered light of pointlike objects. For a wavelength of 543 nm and a specified numerical aperture of 1.4 (oil), we find an axial and lateral focal full width at half-maximum (FWHM) of 460±20 and 145±10 nm, respectively. A high signal-to-noise ratio is obtained by using recording times comparable to those of near-field scanning optical microscopy. We further reduce the effective PSF extent by means of a three-dimensional deconvolution technique exploiting the information gained from the measurement of the focus. We show that it is possible to obtain an axial and lateral FWHM of the far-field effective PSF after deconvolution of 80 and 40 nm, respectively. © 1996 American Institute of Physics. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CONFOCAL microscopy
*PIEZOELECTRIC devices
Subjects
Details
- Language :
- English
- ISSN :
- 00036951
- Volume :
- 69
- Issue :
- 24
- Database :
- Academic Search Index
- Journal :
- Applied Physics Letters
- Publication Type :
- Academic Journal
- Accession number :
- 6289513
- Full Text :
- https://doi.org/10.1063/1.117010