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Nuclear Localization of the δ Subunit of Ca2+ /Calmodulin-Dependent Protein Kinase II in Rat Cerebellar Granule Cells.
- Source :
-
Journal of Neurochemistry . Feb99, Vol. 72 Issue 2, p815-825. 11p. 10 Black and White Photographs, 12 Diagrams, 1 Graph. - Publication Year :
- 1999
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Abstract
- Abstract : To examine the physiological roles of the δ subunit of Ca2+ /calmodulin-dependent protein kinase ∥ (CaM kinase ∥δ) in brain, we examined the localization of CaM kinase ∥δ in the rat brain. A specific antibody to CaM kinase ∥δ1-δ4 isoforms was prepared by immunizing rabbits with a synthesized peptide corresponding to the unique carboxyl-terminal end of these isoforms. The prepared antibody did not recognize the α, β, and γ subunits, which were each overexpressed in NG108-15 cells. Immunoblot analysis on various regions and the nuclear fractions from rat brains suggested that some isoforms of CaM kinase ∥δ1-δ4 were abundant in the nucleus in the cerebellum. Total RNA from the cerebellum was analyzed by RT-PCR with a primer pair from variable domain 1 to variable domain 2. We detected the three PCR products δ3.1, δ3.4, and δ3 that contained the nuclear localization signal. These CaM kinase ∥δ3 isoforms were localized in the nuclei in transfected NG108-15 cells. Immunohistochemical study suggested the existence of these isoforms in the nuclei in cerebellar granule cells. These results suggest that CaM kinase ∥δ3 isoforms are involved in nuclear Ca2+ signaling in cerebellar granule cells. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CALMODULIN
*CEREBELLAR nuclei
Subjects
Details
- Language :
- English
- ISSN :
- 00223042
- Volume :
- 72
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Journal of Neurochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 6270707
- Full Text :
- https://doi.org/10.1046/j.1471-4159.1999.0720815.x