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Secretion of active anti-Ras single-chain Fv antibody by the yeasts Yarrowia lipolytica and Kluyveromyces lactis.

Authors :
Swennen, Dominique
Paul, Marie-Francoise
Vernis, Laurence
Beckerich, Jean-Marie
Fournier, Alain
Gaillardin, Claude
Source :
Microbiology (13500872). Jan2002, Vol. 148 Issue 1, p41-50. 10p. 2 Diagrams, 12 Graphs.
Publication Year :
2002

Abstract

Investigates the secretion vectors of Yarrowia lipolytica and Kluyveromyces lactis for the expression of heterologous proteins. Factors affecting the yield of secretion of soluble single-chain antibody fragment (scFv); Analysis of purified scFv; Capacities of the scFv protein.

Details

Language :
English
ISSN :
13500872
Volume :
148
Issue :
1
Database :
Academic Search Index
Journal :
Microbiology (13500872)
Publication Type :
Academic Journal
Accession number :
6255576