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Aminopropyl-functionalized cubic Ia3d mesoporous silica nanoparticle as an efficient support for immobilization of superoxide dismutase

Authors :
Falahati, Mojtaba
Ma'mani, Leila
Saboury, Ali Akbar
Shafiee, Abbas
Foroumadi, Alireza
Badiei, Ali Reza
Source :
BBA - Proteins & Proteomics. Sep2011, Vol. 1814 Issue 9, p1195-1202. 8p.
Publication Year :
2011

Abstract

Abstract: In this research, the immobilization of superoxide dismutase (SOD) onto aminopropyl-functionalized KIT-6 [n-PrNH2-KIT-6] was investigated. This organo-functionalized mesoporous silica nanoparticle was prepared using a non-ionic surfactant and was fully characterized by XRD, nitrogen adsorption–desorption isotherm assay, IR and TGA techniques. An activity assay demonstrated that the immobilized SOD had a higher activity than the free enzyme. Further investigations using FT-IR, circular dichroism (CD), and probe 1-anilino-8-naphthalene sulfonate (ANS) fluorescence intensity measurements indicated that the structure of the enzyme did not change upon binding to the mesoporous silica, and that immobilized SOD was also less affected by higher temperatures. The melting temperatures of the free and immobilized enzymes were measured by differential scanning calorimetry (DSC), which showed that a fraction of immobilized enzyme was more stable and revealed that immobilized enzyme was partly reversible. [Copyright &y& Elsevier]

Details

Language :
English
ISSN :
15709639
Volume :
1814
Issue :
9
Database :
Academic Search Index
Journal :
BBA - Proteins & Proteomics
Publication Type :
Academic Journal
Accession number :
61921181
Full Text :
https://doi.org/10.1016/j.bbapap.2011.04.005