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Protein identification with Liquid Chromatography and Matrix Enhanced Secondary Ion Mass Spectrometry (LC-ME-SIMS)
- Source :
-
Journal of Proteomics . Jun2011, Vol. 74 Issue 7, p993-1001. 9p. - Publication Year :
- 2011
-
Abstract
- Abstract: Secondary Ion Mass Spectrometry (SIMS) is a well established method for sensitive surface atomic and molecular analysis. Protein analysis with conventional SIMS has been attempted numerous times; however it delivers exclusively fragment peaks assigned to α-amino acids or immonium ions. In this paper we report experiments where direct sequence information could be measured thanks to a combination of HPLC separation with matrix enhanced SIMS (ME-SIMS) on tryptic digests of intact proteins. We employ peptide mass fingerprinting (PMF) and protein identification through the detection of HPLC-separated digests of Savinase (Sav.) and bovine serum albumin (BSA), followed by MASCOT search. This is the first time that the possibility of full protein identification using LC-ME-SIMS is demonstrated in a classic proteomics workflow and that a 69kDa protein is identified with SIMS. These results demonstrate both the relevance and the potential of LC-ME-SIMS in future high resolution proteomics studies. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 18743919
- Volume :
- 74
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- Journal of Proteomics
- Publication Type :
- Academic Journal
- Accession number :
- 61187003
- Full Text :
- https://doi.org/10.1016/j.jprot.2011.02.009