Fast Separation Ultra-Performance Liquid Chromatography for Determination of Pre-Column Derivative Abamectin and Ivermectin Residues in Vegetable.

new residue method for quantification of abamectin and ivermectin in vegetable is described in the article. The derivative process is devised that acylating chemical is firstly performed by N-methylimidazole (MI) and trifluoroacetic anhydride (TFAA), then which reacted with hydroxyl function of abamerctin to make fluorescence. The influence of triethylamin (TEA) is examined. Separation is resolute by a short column of 1.7 μm size and operated at high pressure values (10.000 psi). The optimal chromatographic condition and the highest sensitivity are achieved by acetonitrile: water (95: 5), 0.4 mL/min, 0.2 μL injector. The detection limits of abamectin and ivermectin are 1 μg/kg respectively. [ABSTRACT FROM AUTHOR]