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Suppression subtractive hybridization reveals differential gene expression in sunflower grown in high P
- Source :
-
Plant Physiology & Biochemistry . Jun2011, Vol. 49 Issue 6, p584-591. 8p. - Publication Year :
- 2011
-
Abstract
- Abstract: Sunflower (Helianthus annuus L.) is a commercially important oilseed crop. Previous studies proved that this crop is a promising plant species for phytoextraction of excess soil phosphorus (P) because of its superior P accumulating characteristics. Suppression subtractive hybridization (SSH) strategy was employed to isolate and characterize genes that are induced in response to high P in this crop. SSH library was prepared using cDNA generated from plants treated with high P as the ‘tester’. Based on the results of dot blot analysis, 360 positive cDNA clones were selected from the SSH library for sequencing. A total of 89 non-redundant expressed sequence tags (ESTs) were identified as high P-responsive genes and they were classified into 6 functional groups. Several genes involved in metabolism showed markedly preferential expression in the library. For further confirmation, thirteen of the representative ESTs were selected from all categories for RT-PCR analysis and the results showed up-regulation of these genes in response to high P-treatment. The gene expression data derived from this study suggested that several of the up-regulated genes identified under high P-treatment might be involved in P-accumulation and tolerance in this plant. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 09819428
- Volume :
- 49
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Plant Physiology & Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 60662913
- Full Text :
- https://doi.org/10.1016/j.plaphy.2011.02.017