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Virus — Poly(3,4-ethylenedioxythiophene) Composite Films for Impedance-Based Biosensing.
- Source :
-
Analytical Chemistry . 4/1/2011, Vol. 83 Issue 7, p2420-2424. 5p. - Publication Year :
- 2011
-
Abstract
- Composite films composed of poly(3,4-ethylenedioxythiophene), PEDOT, and the filamentous virus M13-K07 were prepared by electrooxidation of 3,4-ethylenedioxythiophene (EDOT) in aqueous solutions containing 8 nM of the virus at planar gold electrodes. These films were characterized using atomic force microscopy and scanning electron microscopy. The electrochemical impedance of virus-PEDOT films increases upon exposure to an antibody (p-Ab) that selectively binds to the M13 coat peptide. Exposure to p-Ab causes a shift in both real (ZRE) and imaginary (ZIM) impedance components across a broad range of frequencies from 50 Hz to 10 kHz. Within a narrower frequency range from 250 Hz to 5 kHz, the increase of the total impedance (Ztotal) with p-Ab concentration conforms to a Langmuir adsorption isotherm over the concentration range from from 6 to 66 nM, yielding a value for Kd = 16.9 nM at 1000 Hz. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00032700
- Volume :
- 83
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- Analytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 60523658
- Full Text :
- https://doi.org/10.1021/ac2000835