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Evaluation of two internalizing carcinoembryonic antigen reporter genes for molecular imaging.
- Source :
-
Molecular Imaging & Biology . Jun2011, Vol. 13 Issue 3, p526-535. 10p. 3 Diagrams, 2 Graphs. - Publication Year :
- 2011
-
Abstract
- <bold>Purpose: </bold>The objective of this article is to develop internalizing positron emission tomography (PET) reporter genes for tracking genetically modified T cells in vivo.<bold>Procedures: </bold>The transmembrane and cytoplasmic domains of the human transferrin receptor (TfR) and CD5 were each fused to the carcinoembryonic (CEA) minigene N-A3 and expressed in Jurkat T cells. Internalization was evaluated by confocal microscopy or by intracellular uptake of ¹²⁵I-labeled anti-CEA scFv-Fc. Reporter gene-transfected Jurkat xenografts in mice were analyzed by immunohistochemistry (IHC) and imaged by PET using ¹²⁴I- or ⁶⁴Cu-scFv-Fc as tracers.<bold>Results: </bold>Surface expression of TR(1-99)-NA3 was lower than that of NA3-CD5. Both reporter genes were internalized following binding of the anti-CEA antibody fragment. IHC of tumors showed strong staining of NA3-CD5, whereas TR(1-99)-NA3 stained weakly. Specific targeting of TR(1-99)-NA3 or NA3-CD5 was shown by PET in xenografted mice.<bold>Conclusions: </bold>The in vivo imaging studies suggest a potential application of the internalizing form of CEA (N-A3) as a PET reporter gene. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15361632
- Volume :
- 13
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Molecular Imaging & Biology
- Publication Type :
- Academic Journal
- Accession number :
- 60393015
- Full Text :
- https://doi.org/10.1007/s11307-010-0375-0