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Detection of Borrelia burgdorferi DNA in Tick Feces Provides Evidence for Organism Shedding During Vector Feeding.
- Source :
-
Vector-Borne & Zoonotic Diseases . Mar2011, Vol. 11 Issue 3, p197-200. 4p. 2 Charts, 1 Graph. - Publication Year :
- 2011
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Abstract
- Borrelia burgdorferi, the bacterium that causes Lyme disease, is transmitted to a susceptible host by Ixodes spp. tick bites. However, there is uncertainty whether B. burgdorferi are shed from ticks by the fecal route. In this study, B. burgdorferi-infected ticks were fed on mice while confined to a certain area of the skin by a capsule. During and after feeding, tick feces were collected and placed in Barbour-Stoenner-Kelley (BSK)-II media for cultivation and in sterile water for polymerase chain reaction (PCR) analysis. Although none of the tested samples were culture positive for B. burgdorferi, all but one of the fecal DNA samples from infected ticks were PCR positive. These results indicated that B. burgdorferi were shed from feeding ticks during defecation and suggest that the spirochetes did not remain viable once exposed to the outside environment. This finding has important ramifications for investigators interpreting B. burgdorferi-specific PCR results when conducting tick transmission experiments. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15303667
- Volume :
- 11
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Vector-Borne & Zoonotic Diseases
- Publication Type :
- Academic Journal
- Accession number :
- 59652063
- Full Text :
- https://doi.org/10.1089/vbz.2010.0149