ISOLATION AND DETERMINATION OF 4-BIPHENYLAMINE FOR AIR SAMPLE BY SPE-HPLC COUPLING.

The method of determination of 4-biphenylamine (4-BA) in workplace air using SPE and HPLC has been presented. The method consists in passing the air that contains 4-BA through a glass fiber filter with sulfuric acid placed on it, washing out the substance settled on the filter, using water and a solution of sodium hydroxide, solid phase extraction (SPE) in order to enrich the analyte, replace the dissolvent with methanol, and analyze the obtained solution using a method of highly efficient liquid chromatography with fluorescent detection (λex = 268 nm, λem = 389 nm). Measuring range for the 100 dm3 air sample was: 0.1-2 μg/m3. Limit of detection (LOD) and limit of quantification (LOQ) were 0.18 ng/m3 and 0.54 ng/m3, respectively. [ABSTRACT FROM AUTHOR]