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Synthetic circuit identifies subpopulations with sustained memory of DNA damage.
- Source :
-
Genes & Development . 3/1/2011, Vol. 25 Issue 5, p2-2. 1p. - Publication Year :
- 2011
-
Abstract
- Differential responses to stimuli can affect how cells succumb to disease. In yeast, DNA damage can create heterogeneous responses. To delineate how a response contributes to a cell's future behavior, we constructed a transcription-based memory circuit that detects DNA repair to isolate subpopulations with heritable damage responses. Strongly responsive cells show multigenerational effects, including growth defects and iron-associated gene expression. Less-responsive cells exhibit increased mutation frequencies but resume wild-type behavior. These two subpopulations remain distinct for multiple generations, indicating a transmissible memory of damage. Collectively, this work demonstrates the efficacy of using synthetic biology to define how environmental exposure contributes to distinct cell fates. [ABSTRACT FROM AUTHOR]
- Subjects :
- *DNA damage
*DNA repair
*GENE expression
*CELLS
*DNA
Subjects
Details
- Language :
- English
- ISSN :
- 08909369
- Volume :
- 25
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Genes & Development
- Publication Type :
- Academic Journal
- Accession number :
- 59393498
- Full Text :
- https://doi.org/10.1101/gad.1994911